Biochemistry: How Fats Enter the Powerhouse! Beta oxidation of fatty acids happens in the Mitochondrial Matrix, but there's a catch: Long Chain Fatty Acids (LCFAs) cannot cross the inner mitochondrial membrane on their Carnitine Shuttle an overview ScienceDirect Topics The Importance of the Fatty Acid Transporter L Carnitine in Non Alcoholic Fatty Liver Disease (NAFLD) Metabolism: Master the Carnitine Shuttle and Stop Exam Stress! Long Chain Fatty Acids (LCFAs) love to burn energy, but they face a major obstacle: they cannot enter the mitochondrial matrix where Beta Oxidation Carnitine transport and fatty acid oxidation ScienceDirect