Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health The Use and Potential Benefits of N Acetylcysteine in Non Acetaminophen Acute Liver Failure: An Etiology Based Review Glutathione You Are The Healer Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome Scientific Reports Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.