Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Tylenol's Impact on Glutathione and Liver Damage Acetaminophen or Tylenol Toxicity Lect 19 Pharmacology Acetaminophen metabolism Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.